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海洋科学  2007 

几种重组别藻蓝蛋白的抗氧化活性

, PP. 71-75

Keywords: 重组别藻蓝蛋白,清除自由基,2,2-盐酸脒基丙烷(AAPH),藏红花素

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Abstract:

藻胆蛋白是某些藻类特有的捕光色素蛋白,其中天然别藻蓝蛋白、藻蓝蛋白的抗氧化活性已经被证明。实验以2,2-盐酸脒基丙烷(AAPH)为自由基生成者,应用藏红花素退色反应为检测清除氢过氧自由基的方法,探讨了在大肠杆菌(Escherichiacoli)中表达并纯化的带有6×His(6×组氨酸)标签和带有麦芽糖结合蛋白(MBP)标签的重组别藻蓝蛋白及其α、β亚基的抗氧化活性。结果表明,带有His标签的6×His-αAPC、6×His-βAPC和6×His-APC均显示出一定的清除氢过氧自由基的能力,其中6×His-βAPC清除氢过氧自由基的IC50值可达到27.2mg/L,Ka/Kc(氢过氧自由基与重组别藻蓝蛋白和藏红花素反应的速度常数比)达到1.24,大于带有色素基团的天然APC清除氢过氧自由基能力(IC5057.5mg/L);而带有MBP标签的重组别藻蓝蛋白亚基MBP-αAPC和MBP-βAPC和MBP-APC(rAPC)则无明显的清除能力。结果首次证实了脱辅基蛋白具有清除氢过氧自由基能力,因而在天然藻胆蛋白中脱辅基蛋白是清除自由基的贡献者之一,这为进一步研究重组别藻蓝蛋白的抗肿瘤机理提供了线索。

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