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海洋科学  2007 

皮状丝孢酵母利用大米草水解液发酵生产微生物油脂

, PP. 38-41

Keywords: 皮状丝孢酵母(Trichosporon,cutaneum),大米草(Spartina,anglica),水解,发酵,微生物油脂

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Abstract:

研究了产油酵母菌皮状丝孢酵母(Trichosporoncutaneum)利用经过简单脱毒处理的大米草水解液,直接发酵生产微生物油脂。首先分别用1%、2%、4%和6%稀硫酸,在122℃水解大米草,大米草/硫酸(固/液比)为10%。结果表明,在6%的酸浓度下水解40min后总还原糖含量最高。而1%稀硫酸在140℃下水解大米草,2.8h后,水解液中葡萄糖产量达到最高14.2g/L。然后将该条件下大米草水解液冷冻干燥浓缩为不同的倍数,作为皮状丝孢酵母菌的发酵培养基,水解液浓缩倍数为4.0时,产油量最高达6.0g/L。为高效利用大米草,将大米草开发为一种能源植物,提供了新的途径。

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