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海洋科学  2007 

超低温保存时间与抗冻剂浓度对真鲷精子质量的影响

, PP. 85-87

Keywords: 真鲷(Pagrus,major),超低温保存,抗冻剂浓度,二甲基亚砜(DMSO),保存时间,受精力

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Abstract:

研究了不同浓度的抗冻剂二甲基亚砜(DMSO)和不同保存时间对真鲷(Pagrusmajor)冻精质量的影响。试验结果表明,DMSO体积分数和保存时间对冻精的受精率、孵化率影响显著。15%二甲基亚砜作为抗冻剂保存10~60d的冻精受精率、孵化率与鲜精无差异,而保存360d的冻精受精率、孵化率开始出现下降。12%,18%,21%DMSO保存60d的冻精受精率差异不显著,均高于90%,而对于保存360d的冻精受精率、孵化率均显著地降低。

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