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海洋科学  2007 

大黄鱼精液的高效超低温保存

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Keywords: 超低温保存,抗冻剂,大黄鱼(Pseudosciaena,croce),精液,运动率,受精率

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Abstract:

采用2mL的冷存管和程序降温仪高效超低温保存大黄鱼(Pseudosciaenacroce)精液。分析比较了5种不同浓度抗冻剂(二甲基亚砜、乙二醇、丙二醇和甘油和甲醇)、3种降温速率(10,20和30℃/min)和2种解冻温度(28℃和43℃)对大黄鱼精液超低温保存效果的影响。实验结果表明,采用Hanks’作为稀释液,10%~20%甘油或10%DMSO作为抗冻剂,20℃/min的降温速率对精液进行超低温保存43℃水浴解冻,冻精激活后获得理想的运动率(76.6%±11.4%~80.0%±12.6%),而且对10%甘油保存的冻精进行人工授精实验获得了与新鲜精液相似的受精率和孵化率。

References

[1]  Gwo J C,Ohta H,Okuzawa K,et al.Cryopreservation of sperm from the endangered formosan landlocked salmon (Oncorhynchus masou formosanus)[J].Theriogenology,1999,51:569-582.
[2]  Tiersch T R,Figiiel C R,Jr W R,et al.Cryopreservation of sperm of the endangered razorback sucker[A].Tiersch T R,Mazik P M.Cryopreservaation in aquatic species[C].Baton Rouge,Louisiana,USA:World Aquaculture Society,2000.117-122.
[3]  Liu Q H,Li J,Zhang S C,et al.An efficient ethodology of spermatozoa cryopreservation with 2-mL cryovials in red seabream Pagrosomus major[J].Journal of the World Aquaculture Society,2006,37:289-297.
[4]  李纯,李军,薛钦昭.真鲷精液超低温保存研究[J].海洋科学,2001,12:6-8.
[5]  李纯,李军,薛钦昭.黑鲷精液超低温保存研究[J].海洋科学,2001,11:1-4.
[6]  Chen S L,Ji X S,Yu G C,et al.Cryopreservation of sperm from turbot (Scophthalmus maximus) and application to large-scale fertilization[J].Aquaculture,2004,236:547-556.
[7]  Zhang Y Z,Zhang S C,Liu X Z,et al.Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology[J].Theriogenology,2003,60:989-996.
[8]  洪万树,张其永,许胜发,等.花鲈精子生理特性及其精液超低温冷冻保存[J].海洋学报,1996,18:97-104.
[9]  Linhart O,Billard R,Proteau J P.Cryopreservation of European catfish (Silurus glanis L.) spermatozoa[J].Aquaculture,1993,115:347-359.
[10]  Young J A,Capra M F,Blackshaw A W.Cryopreservation of summer whiting (Sillago ciliata) spermatozoa[J].Aquaculture,1992,102:155-160.
[11]  Rideout R M,Litvak M K,Trippel E A.The development of a sperm cryopreservation protocol for winter flounder,Pseudopleuronectes americanus (Walbaum):evaluation of cryoprotectants and diluents[J].Aquaculture,2003,34:653-659.
[12]  林丹军,尤永隆.大黄鱼精子生理特性及其冷冻保存[J].热带海洋学报,2002,21:69-75.
[13]  Gwo J C.Cryopreservation of yellowfin seabream (Acanthopagrus latus) spermatozoa(teleost,perciformes,sparides)[J].Theriogenology,1994.41:989-1 004.
[14]  Gwo J C,Strawn K,Longnecker M T,et al.Cryopreservation of Atlantic croaker spermatozoa[J].Aquaculture,1991,94:355-375.
[15]  Dr anno C,Suquet M,Quemener L,et al.Cryopreservation of turbot (Scophthalmus maximus) spermatozoa[J].Theriogenology,1997,48:589-603.
[16]  Denniston R S,Michelet S,Godke R A.Principles of Cryopreservation[A].Tiersch T R,Mazik P M.Cryopreservation in aquatic species[C].Baton Rouge,Louisiana,USA:World Aquaculture Society,2000.59-74.

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