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海洋科学  2006 

合浦珠母贝β-肌动蛋白基因的克隆和序列分析

, PP. 64-70

Keywords: 合浦珠母贝(Pinctada,fucata),β-肌动蛋白,序列分析,进化

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Abstract:

为进一步研究珍珠质基质蛋白外排机制,同时为今后合浦珠母贝(Pinctadafucata)不同组织的基因表达研究提供阳性对照,根据大鼠(Rattusnorvegicus)胞质型肌动蛋白β-actin设计引物,通过RT-PCR方法克隆鉴定了合浦珠母贝肌动蛋白同源基因片段。序列分析表明,该片段编码的氨基酸序列与相应同源片段具有较高的保守性。合浦珠母贝β-肌动蛋白有多个特有的氨基酸残基。合浦珠母贝可能是在系统进化上较早分出的一支。

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