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海洋科学  2006 

雨生红球藻β-胡萝卜素酮化酶基因的cDNA和基因组DNA克隆及序列分析

, PP. 20-27

Keywords: 雨生红球藻(Haematococcus,pluvialis),β-胡萝卜素酮化酶,cDNA,基因组DNA,克隆,序列分析

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Abstract:

根据已知的β-胡萝卜素酮化酶(β-caroteneketolase,BKT)的cDNA序列设计引物,利用长距离PCR扩增获得了bkt基因的cDNA完整编码片段及基因组DNA片段,并对这些片断进行了序列测定。cDNA和基因组DNA比对结果表明该基因至少包括5个内含子,它们的剪切位点皆符合GU-AG规律。在比对结果中同时还发现一个19bp的短序列重复,该重复序列在bkt的cDNA和基因组DNA上都发生了多次重复,推测在BKT的表达调控中可能具有一定功能。另外,在序列比对过程中还发

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