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海洋科学  2006 

克氏原螯虾热休克蛋白70ku类似物基因的cDNA分析

, PP. 31-34

Keywords: 全长cDNA,快速扩增cDNA末端,同源性,70ku热休克蛋白同类物

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Abstract:

根据已测序的克隆号PCI246基因序列设计2条引物,用快速扩增cDNA末端技术扩增克氏原螯虾(Procambarusclarkii)70ku热休克蛋白同类物基因cDNA的5’端片段和3’端片段,测序得到1184bp基因片段,包含该基因的完整3’端,GenBank登录号AY357302,与GenBank序列号AB016836家蚕(Bombyxmori)的70ku热休克蛋白同类物mRNA的部分片段83%同源。和果蝇(Drosophilamelanogaster)的热休克蛋白同类物3的同源性最高(GenBank的登录号分别是NP_727563,NP_511132,NP_727564,NP_727565),为85%。

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