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海洋科学  2006 

海链藻目核糖体RNA基因片段的扩增及多样性分析

, PP. 40-44

Keywords: 海链藻目(Thalassiosirales),18S核糖体RNA基因,多样性

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Abstract:

海链藻目(Thalassiosirales)的海链藻属(Thalassiosira)和骨条藻属(Skeletonema)微藻是形成春季赤潮的主要种类。为利用分子生物学方法分析表层海水主要赤潮形成藻的动态变化、快速鉴定赤潮形成藻种,设计了海链藻目特异引物,扩增了胶州湾近岸表层海水海链藻目18S核糖体RNA基因片断。随机测定10个序列,其中4个与海链藻属的Thalassiosiraconcaviuscula的完全相同,其余的可确定属于海链藻属。表明该引物对能选择性地扩增海链藻目18S核糖体RNA基因片断。

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