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海洋科学  2008 

海洋聚磷菌中核苷二磷酸激酶基因的克隆及序列分析

, PP. 61-63

Keywords: 盐单胞菌属,聚磷菌,核苷二磷酸激酶(NDPK)

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Abstract:

以海洋聚磷茵HalomonasYSR-3的总DNA为模板,用PCR法扩增核苷二磷酸激酶基因,将扩增片段克隆到pGM-T载体,转化EscherichiacoliTOP10菌株,经蓝白斑筛选、菌落PCR得到阳性克隆,测序后对序列进行Blast比对分析。得到的基因序列长度为420bp,翻译后的序列与LoktanellayestfoldensisSKA53,Jannaschiasp.CCS1,Roseobact

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