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海洋科学  2008 

富含古罗糖醛酸的褐藻多糖生产菌株的构建

, PP. 14-18

Keywords: 甘露糖醛酸C-5差向异构酶,假单胞菌,褐藻多糖,过量表达

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Abstract:

为获得古罗糖醛酸(Guluronate)含量高的细菌胞外褐藻多糖,利用PCR从海洋细菌Pseudomonassp.QDA中克隆了其甘露糖醛酸C-5差向异构酶基因(algG),连接入质粒pMF54Km,构建了重组表达载体pMF54Km-algG。利用三亲接合法将pMF54Km-algG转入菌株QDA中,获得algG过量表达重组菌株QDA-G。1H-NMR测定结果表明,QDA-G所产的褐藻多糖中β-D-甘露糖醛酸(M)与它的C-5差向异构体α-L-古罗糖醛酸(G)的比值为0.38,G的质量分数达到74.2%,比野生菌株QDA提高了26.4%。且重组菌株遗传稳定性良好,连续传代20代后,M/G的比值无明显变化。

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