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海洋科学  2009 

产重组别藻蓝蛋白类荧光蛋白的重组大肠杆菌发酵条件的优化

, PP. 57-61

Keywords: 重组别藻蓝蛋白类荧光蛋白,发酵,优化

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Abstract:

对重组别藻蓝蛋白类荧光蛋白基因工程菌在装有150mL液体培养基的250mL摇瓶中的发酵条件进行优化。对起始pH值、接种量、诱导起始时间、诱导温度和时长以及IPTG浓度对重组荧光蛋白表达量进行了分析。结果表明,在装有150mL液体培养基的250mL摇瓶中,在起始pH值为7.5,6%接种量,培养温度为37℃,培养时长为3~5h,诱导温度为22℃,IPTG浓度为0.2mmol/L,诱导时长为7~8h的条件下发酵重组别藻蓝蛋白类荧光蛋白,其表达量最高。

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