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海洋科学  2009 

大菱鲆卵黄原蛋白的分离纯化及Native-PAGE、SDS-PAGE性质鉴定

, PP. 71-75

Keywords: 大菱鲆(Scophthatmus,maximus),卵黄原蛋白,分离纯化,Native-PAGE,SDS-PAGE

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Abstract:

采用5mg/kg17β-雌二醇对大菱鲆(Scophthalmusmaximus)进行肌肉注射,一周后尾静脉取血,离心分离获得血浆,经SephacrylS-300(高分辨)分子筛纯化卵黄原蛋白,对卵黄原蛋白进行常规聚丙烯酰胺凝胶电泳,考马斯亮蓝染色和糖、磷、脂的特征性基团染色。结果表明,17β-雌二醇能诱导大菱鲆产生卵黄原蛋白,以Native-PAGE方法计算得到大菱鲆卵黄原蛋白的分子质量为538ku,以SDS-PAGE方法得出其两种亚基的分子质量分别为100ku和82ku。分子筛凝胶过滤能较好地纯化大菱鲆血浆中的卵黄原蛋白。

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