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海洋科学  2009 

盐田藻垫细菌的分离及其胞外多糖的初步研究

, PP. 63-67

Keywords: 盐田,藻垫,细菌,胞外多糖

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Abstract:

从连云港台南盐场采集藻垫,在去除底泥等杂物的藻类层中分离出5株不同的细菌(编号为A,B,C,D和E)。经VITEK-32鉴定,A和B可能是鲁氏不动杆菌(Acinetobacterlwoffii)或琼氏不动杆菌(Acinetobacterjunii),C为溶血巴斯德菌(Pasteurellahaemolytica),D为尿素放线杆菌(Actinobacillusureae),E未鉴定出结果。在所试验的盐度范围内,细菌A、B和C的最适生长盐度都为25,细菌D的最适生长盐度为50~100,细菌E的最适生长盐度为25~50。细菌A,B,C,D和E细菌胞外多糖产率分别为0.628,0.362,0.177,0.269,0.719g/g细胞干质量。细菌A,B,C,D和E胞外多糖的糖醛酸质量分数分别为5.4%,9.9%,7.4%,9.8%和23.4%,均不含蛋白质,均无乳化、絮凝及凝胶活性。实验结果显示细菌胞外多糖是构成盐田藻垫胶质的重要组成部分,但从其物化性质看,细菌胞外多糖对藻垫的胶结和黏附性没有直接的影响。

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