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海洋科学  2010 

长期超低温保存后真鲷精子的质量变化

, PP. 50-54

Keywords: 低温保存,真鲷,(Pagrus,major),精子,运动率,受精率

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Abstract:

通过对精子运动率、受精率和超微结构的观察,研究了2003~2009年分5批冷冻保存的真鲷(Pagrusmajor)精子经1~73个月保存后质量变化情况。冷冻精子在40℃水浴中解冻,大约100~110s转化为液态后取出,用海水激活后观察精子运动率,人工受精6~8h后观察受精率,同时精子用2.5%戊二醛前固定,经处理后用扫描电镜和透射电镜观察精子超微结构。结果表明,冷冻精子的最高运动率(87.67%±2.52%)和受精率(71.33%±8.84%)都是2009年保存1个月的精子,保存1,13,26个月的精子运动率和受精率差异不显著(P<0.05),保存48个月后的精子运动率和受精率显著低于保存1个月的精子(P<0.05),2003年保存的精子(73个月)运动率(50.67%±5.31%)和受精率最低(39.56%±0.69%)。精子的超微结构损伤主要包括精子头部损伤,线粒体损伤以及精子质膜的损伤。

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