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一株降解纤维素梭形芽胞杆菌的筛选与鉴定

Keywords: 降解纤维素,筛选,鉴定,梭形芽胞杆菌

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Abstract:

从肥沃的土壤中经过分离获得14株降解纤维素的细菌,以菌株在纤维素刚果红平板中溶菌圈直径与菌落直径的比值为依据进行筛选,获得一株比值为4.5的菌株,编号为X62。采用DNS法对影响该菌产生纤维素酶活力的单因素进行了分析,并通过对其进行形态特征、生理生化特征测定以及16SrDNA序列分析,确定X62为梭形芽胞杆菌(Lysinibacillusfusiformis),将其命名为LysinibacillusfusiformisX62。X62的16SrRNA序列GenBank登录号为JX198550。

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