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红三叶草根际区石油降解菌的筛选及降解性能

Keywords: 石油降解菌,根际区,筛选,鉴定,降解性能

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Abstract:

从石油污染的土壤红三叶草(TrifoliumrepensLinn)根际修复区中分离筛选得到4株以原油作为惟一碳源和能源进行生长繁殖的高效石油降解菌。通过菌落形态、显微镜个体形态观察、生理生化鉴定以及菌株16SrDNA序列分析,初步鉴定4株优势降解菌分别为动性杆菌、藤黄微球菌、蜡状芽孢杆菌和短小芽孢杆菌。采用气相色谱/质谱(GC/MS)法分析4株混合菌对石油烃的降解性能。结果表明:在摇床培养条件下,混合菌54d对总石油烃的生物降解率达到90.50%,较对照高67.72%。随着生物降解时间的延长,石油组分中的正构烷烃、异构烷烃及环烷烃相对总量均呈减小趋势,而芳香烃和其他醇类、醛和酸类的相对含量则有所增加。

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