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一种未见报道的厌氧氨氧化菌分子生物学鉴定

Keywords: 厌氧氨氧化菌,16S,rDNA,鉴定

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Abstract:

以(NH4)2SO4和NaNO2作为基质,富集厌氧氨氧化污泥。提取厌氧氨氧化污泥中细菌总DNA,纯化后使用特异性引物对厌氧氨氧化菌16SrDNA进行PCR扩增。扩增产物连接到pMD19-T载体,将载体转化到感受态细胞大肠杆菌JM109中,并对其16SrDNA基因进行测序。将测序结果进行系统发育树分析,发现富集得到的厌氧氨氧化菌与CandidatusAnammoxoglobuspropionicus进化关系比较接近,是一种尚未见报道的厌氧氨氧化菌。

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