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药渣残留泰乐菌素的酶促降解特性

Keywords: 药渣,无丙二酸柠檬酸杆菌,泰乐菌素,降解酶

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Abstract:

发酵法生产泰乐菌素过程中产生的药渣,因残留泰乐菌素的存在,会对环境造成不利影响。采用富集驯化方法,从堆放泰乐菌素药渣附近土壤中筛选到1株高效降解泰乐菌素的菌株,经16SrDNA鉴定为无丙二酸柠檬酸杆菌(Citrobacteramalonaticus)。该菌产生的降解泰乐菌素的主要酶是胞内组成酶。单因素实验结果显示,该酶降解泰乐菌素适宜的条件为温度35℃、pH5.5、加酶量12%和初始底物浓度低于20mg/L。多因素正交实验结果显示,温度对泰乐菌素的降解率影响较大。

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