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不同质量浓度苦草对铜绿微囊藻生长及抗氧化酶系统的影响

Keywords: 苦草,铜绿微囊藻,共生培养,化感物质

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Abstract:

采用苦草(VallisneriaspiralisLinn.)和铜绿微囊藻(Microcystisaeruginosa)共生培养的实验方法,通过追踪测定铜绿微囊藻的生物量、叶绿素a含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性,研究了不同质量浓度苦草对铜绿微囊藻生长及抗氧化酶系统的影响。结果表明,质量浓度大于10g/L时,苦草对铜绿微囊藻有明显的抑制作用,表现为苦草质量浓度为10、20和40g/L时,第15天对铜绿微囊藻的抑制率分别为63.3%、94.7%和99.8%,培养过程中,铜绿微囊藻的叶绿素a含量逐渐减少,而SOD、POD活性及MDA含量呈现先增加后逐渐降低的趋势,表明苦草释放的化感物质在经过一定时间积累后能够明显抑制铜绿微囊藻SOD和POD的活性,引起细胞的氧化损伤,促进叶绿素的分解,从而导致藻类死亡,这是苦草抑制铜绿微囊藻生长的原因之一。

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