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处理畜禽废水的序批式生物膜反应器中的细菌多样性

Keywords: SBBR,畜禽废水,细菌多样性,PCR-DGGE,系统发育树

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Abstract:

采用序批式生物膜反应器(SBBR)处理畜禽废水,在室温(9~18℃)下,采用8h/周期、交替停曝气的模式运行,控制曝气阶段DO浓度在2mg/L,可实现明显的亚硝酸盐积累,氨氮及总氮的去除率分别可达(95.1±0.8)%和(87.2±0.6)%。为揭示SBBR中细菌种群构成及其动态变化规律,采用PCR-DGGE技术进行了细菌多样性分析,并构建了系统发育树,结果表明:与接种污泥相比,驯化期生物膜中细菌种群丰富度未发生明显变化,运行期交替曝气、停曝模式有助于提高生物膜中细菌的多样性指数,但受运行模式及氨氮负荷变化影响,运行期氨氧化菌多样性指数略低于驯化期;生物膜内存在一些具有反硝化功能的变形菌和特殊的氨氧化细菌,在本实验条件下未发现厌氧氨氧化菌,说明主要脱氮机理为同时短程硝化反硝化。

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