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A+OSA污泥减量工艺的微生态特性

Keywords: OSA工艺,剩余污泥减量,微生物群落结构,PCR-DGGE

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Abstract:

采用16SrDNA序列与PCR-DGGE(polymerasechainreaction-denaturinggradientgelelectrophoresis)分析技术相结合的方法,研究了A+OSA(theanoxic+oxic-settling-anaerobic)污泥减量工艺在不同工况下的减量效果及其微生态特性。结果显示,在自然条件下,A+OSA工艺可有效减少剩余污泥27%左右。分子生物研究表明,解耦联池的插入可以明显改变系统微生物的群落结构,且随着解耦联池水力停留时间的延长,系统中部分微生物被“淘洗”,微生物丰富度和多样性指数均有所降低。相似性分析表明,参照系统和A+OSA工艺分属于2个不同的集群,但在A+OSA工艺内部各反应池样品间具有较高的相似性,且各反应池在HRT为5.16h和7.14h时,表现为显著相似。通过上述研究可为该工艺优化及调控提供理论指导。

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