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耐盐性毒死蜱降解混合菌的构建及其降解特性

Keywords: 生物降解,混合菌,耐盐性,蜡状芽孢杆菌,毒死蜱

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Abstract:

为明确蜡状芽孢杆菌(Bacilluscereus)混合菌株对毒死蜱的降解效果,采用正交实验的方法构建混合菌。以混合菌对毒死蜱的降解率和菌株的生长量为依据,利用单一因素实验考察了不同因素对混合菌降解毒死蜱的影响。结果表明:构建的混合菌中三菌株的体积比为1∶1∶3。在含80mg/L毒死蜱的反应体系中,最适接菌量为8%(V/V),最适pH为7。在实验浓度下,混合菌对毒死蜱的降解符合一级动力学方程。混合菌对盐分有较高的耐受度,当反应液中氯化钠浓度在20~100g/L之间时,混合菌对80mg/L毒死蜱的降解率最高达61%。

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