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抗铬菌株的筛选鉴定及其生物学特性和吸附特性

Keywords: 抗铬菌株,筛选鉴定,生物学特性,吸附特性

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Abstract:

利用微生物分离纯化技术,在培养基中加入不同浓度的Cr3+、Cr6+,经过长时间驯化,从污染土壤中筛选出2株对Cr具有较高抗性的菌株(A和B),对其进行了形态、生理生化特性及分子生物学鉴定,并研究了其最佳生长条件及对Cr3+的生物吸附规律。结果表明,菌株A和B分别为蜡状芽孢杆菌(Bacilluscereus)和米根霉菌(Rhizopusoryzae)。菌株A和B的最适生长pH值均为7,最适生长温度均为30℃,最佳装液量为80~100mL(250mL摇瓶)。在培养时间一定时,随着Cr3+浓度的增加,菌株对Cr3+的吸附率减少,而生物吸附量则逐渐增大;在Cr3+浓度一定时,随着培养时间的延长,菌体对Cr3+的吸附率呈现出先增加后减少的趋势,这与生物吸附量的变化趋势基本一致。在Cr3+浓度为100mg/L时,菌株A和B对Cr3+的吸附率达到最大,分别为51.47%和46.36%。

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