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纺锤芽孢杆菌(Bacillus fusiformis)降解萘的特性及动力学

Keywords: ,微生物降解,纺锤芽孢杆菌,红外光谱,紫外可见光谱

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Abstract:

在前期研究中发现,纺锤芽孢杆菌(Bacillusfusiformis,BFN)可以用于降解水溶液的萘,为了解其降解过程,发现BFN菌生长量随着溶液中的萘的含量增加而提高。其中,萘的含量分别是30、50、100和200mg/L时,BFN的生物量OD600值分别为0.057、0.081、0.126和0.193;降解培养基溶液COD的去除率分别为59.4%、65.3%、69.2%和70.6%,说明BFN菌在生长的过程中利用萘作为碳源。同时,动力学拟合发现,对不同含量萘的降解过程都符合一级降解动力学方程,且BFN菌的生长过程满足逻辑斯蒂方程。扫描电镜图表明,BFN菌在萘的存在下生长得更好。紫外光谱显示波长为276nm的萘的吸收峰在降解后下降很多。红外光谱数据则表明,降解液中有2组新的吸收峰出现:一组出现在2878、2930和2968cm-1处,说明在萘的降解过程中有新的羧酸类生成;另一组出现在3438、3667和3731cm-1处有新的酚类物质生成。

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