全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

好氧反硝化苯酚降解菌的分离鉴定及动力学

Keywords: Diaphorobacter,sp.,PDB3,反硝化,苯酚,生物降解,动力学

Full-Text   Cite this paper   Add to My Lib

Abstract:

从驯化菌群中分离筛选出一株好氧反硝化苯酚降解细菌,经生理生化反应及16SrDNA测序,鉴定为Diaphorobacter属细菌。在好氧条件下,该菌株以苯酚为唯一碳源和能源,利用NO3--N作为反硝化电子受体,其生长与反硝化特性研究表明:在接种量5%(体积分数),30℃,180r/min振荡培养条件下,菌株降酚能力可达1400mg/L,同时,能有效去除初始浓度为165mg/L的硝酸盐氮,60h其去除率为91.5%,高含量苯酚对菌体生长有一定的抑制作用。应用Haldane方程对其生长过程进行动力学模拟,拟合曲线与实验测定值相关性良好,各参数分别为μmax(最大比增长率)0.324h-1,Ks(半饱和常数)9.36mg/L,Ki(抑制常数)146.72mg/L,通过理论分析及实验验证得,该菌株苯酚降解动力学与其生长动力学表现出相似的趋势。

References

[1]  Wei Gehong, Yu Jianfu, Zhu Yuhua,et al. Characterization of phenol degradation by Rhizobium sp.CCNWTB 701 isolated from Astragalus chrysopteru in mining tailing region. Journal of Hazardous Materials, 2008, 151(1): 111-117
[2]  Lu Daban, Zhang Yan, Niu Shiquan, et al. Study of phenol biodegradation using Bacillus amyloliquefaciens strain WJDB-1 immobilized in alginate-chitosan-alginate (ACA) microcapsules by electrochemical method. Biodegradation,2012,23(2):209-219
[3]  徐夫元,李星,陈英文,等.无机阴离子对CuO-H2O2氧化苯酚废水的影响.环境科学,2009,30(2):451-456 Xu Fuyuan, Li Xing, Chen Yingwen, et al. Influences of inorganic anions on oxidation of phenol by CuO-H2O2. Environmental Science, 2009,30(2):451-456(in Chinese)
[4]  张芳,李光明,盛怡,等.三维电解法处理苯酚废水的粒子电极研究. 环境科学,2007,28(8):1715-1719 Zhang Fang, Li Guangming, Shen Yi, et al. Preparation of poarticle-electrodes for treating phenol wastewater using three-dimensional electrolysis. Environmental Science, 2007,28(8):1715-1719(in Chinese)
[5]  Li X. Y.,Cui Y. H.,Feng Y. J.,et al.Reaction pathways and mechanisms of the electrochemical degradation of phenol on different electrodes. Water Research, 2005,39(10):1972-1981
[6]  陈春, 李文英, 吴静文, 等. 焦化废水中苯酚降解菌的筛选及其降解性能.环境科学, 2012,33(5):1652-1656 Chen Chun, Li Wenying, Wu Jingwen, et al. Screening and characterization of phenol degrading bacteria for the coking wastewater treatment. Environmental Science, 2012,33(5):1652-1656(in Chinese)
[7]  Saravanan P., Pakshirajan K.,Saha P.Biodegradation of phenol and m-cresol in a batch and fed batch operated internal loop airlift bioreactor by indigenous mixed microbial culture predominantly Pseudomonas sp. Bioresource Technology, 2008,99(18): 8553-8558
[8]  Semple K. T., Cain R. B. Biodegradation of phenol by the alga Ochrornonus danica. Applied Environmental Microbiology, 1996, 162(4):1256-1273
[9]  Aditi Banerjee,Aloke K.,Ghoshal. Isolation and characterization of hyper phenol tolerant Bacillus sp. from oil refinery and exploration sites. Journal of Hazardous Materials, 2010,176(1-3):85-91
[10]  Latha S., Mahadevari A. Role of rhizobia in the degradation of aromatic substances. World Journal of Microbiology & Biotechnology,1997, 13(6):601-607
[11]  Kalin M., Neujahr H. Y., Weissnahr R. N. Phenol hydroxylase from Trichosporon cutaneum: Gene cloning, sequence analysis, and functional expression in Escherichia coli. Journal of Bacteriol., 1992,174(2):7112-7120
[12]  Huang H. K., Tseng S. K. Nitrate reduction by Citrobacter diversus under aerobic environment. Apply Microbiol. Biotechnology,2001,55(1): 90-94
[13]  Scholten E., Lukow T., Auling G., et al. Thauream echernichensis sp. nov., an aerobic denitrifier from a leachate treatment plant. International Journal of Systematic Bacteriology, 1999, 49(3): 1045-1051
[14]  Robertson L. A., van Niel E. W., Torremans R. A., et al. Simultaneous nitrification and denitrification in aerobic chemostat cultures of Thiosphaera Pantotropha. Applied and Environmental Microbiology, 1988,54(11):2812-2818
[15]  Patureau D., Godon J. J., Dabert P., et al. Microvirgula aerodenitrificans gen. nov., sp. nov., a new gram-negative bacterium exhibiting co-respiration of oxygen and nitrogen oxides up to oxygen-saturated conditions. International Journal of Systematic Bacteriology,1998,48(3): 775-782
[16]  Sandhu Amarjyoti, Halverson Larry J., Beattie Gwyn A. Bacterial degradation of airborne phenol in the phyllosphere. Environ. Microbiol. 2007, 9(2):383-392
[17]  马放,任南琪,杨基先. 污染控制微生物学实验.哈尔滨: 哈尔滨工业大学出版社,2002
[18]  戚以政,汪叔雄. 生化反应动力学与反应器.北京:化学工业出版社,1992
[19]  李亚新. 活性污泥法理论与技术. 北京:中国建筑工业出版社, 2007
[20]  国家环境保护总局.水和废水监测分析方法(第4版).北京:中国环境科学出版社, 2002
[21]  张凤君,戴宁,李卿,等. 活性污泥法驯化筛选好氧反硝化菌的试验研究. 环境科学学报,2006,26(11):1084-1088 Zhang Fengjun,Dai Ning,Li Qing, et al. Study on acclimating and filtrating the aerobic denitrifying bacteria from activated sludge. Acta Scientiae Circumstantiae, 2006,26(11):1084-1088(in Chinese)
[22]  Zhou Y., Pijuan M., Yuan Z. G. Free nitrous acid inhibition on anoxic phosphorus uptake and denitrfication by poly-phosphate accumulating organisms. Biotechnology and Bioengineering, 2007,98(4):903-912
[23]  左薇. 一株好氧反硝化菌的筛选鉴定及其脱氮特性分析. 哈尔滨: 哈尔滨工业大学硕士学位论文,2006 Zuo Wei. An aerobic denitrifier screened identification anddenitrification characteristic. Harbin: Master\'s Degree Thesis of Harbin Institute of Technology,2006 (in Chinese)
[24]  杨基先, 高珊珊, 马放,等.一株好氧反硝化细菌的分离鉴定及反硝化能力. 环境科学学报, 2008,28(7):1302-1307 Yang Jixian, Gao Shanshan, Ma Fang,et al. Identification and phylogenetic analysis of an isolated aerobic denitrifier. Acta Scientiae Circumstantiae,2008,28(7):1302-1307(in Chinese)
[25]  Gupta A. B. Thiosphaera Pantotropha a sulphur bacterium capable of simultaneous heterotrophic nitrification and aerobic denitrification. Enzyme and Microbial Technology, 1997, 21(8): 589- 595
[26]  Jiang Yan, Wen Jianping, Bai Jing. Biodegradation of phenol at high initial concentration by Alcaligenes faecalis. Journal of Hazardous Materials,2007,147(1-2):672-676
[27]  Wang G. Y., Wen J. P., Yu G. H., et al. Anaerobic biodegradation of phenol by Candida albicans PDY-07 in the presence of 4-chlorophenol. Microbiol. Biotechnol.,2008, 24(11):2685-2691
[28]  唐春燕,李巍,叶秋霞,等. 恶臭假单胞菌降解苯酚的动力学研究. 环境工程学报, 2011, 5(10): 2364-2368 Tang Chunyan, Li Wei, Ye Qiuxia, et al. Growth kinetics and phenol biodegradation of Pseudomonas putida LY1. Chinese Journal of Environmental Engineering, 2011, 5(10): 2364-2368(in Chinese)
[29]  杨素亮, 姜岩, 闻建平. 一株高效苯酚降解菌的分离鉴定及动力学研究. 化学工业与工程,2006,23(4):287-290 Yang Suliang, Jiang Yan, Wen Jianping. Isolation and identification of a phenol-degrading yeast with high activity and its kinetic study. Chemical Industry and Engineering,2006,23(4):287-290 (in Chinese)

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133