全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

一株苯酚降解菌的筛选及降解动力学特性

Keywords: 苯酚,生物降解,降解动力学

Full-Text   Cite this paper   Add to My Lib

Abstract:

利用富集驯化的培养方法,从首钢焦化厂废水处理系统中的二沉池出水中,分离筛选出一株能够高效降解苯酚的菌株B3对其16SrDNA序列进行分析,并选择Monod方程和Andrews方程分别研究该菌在不同苯酚浓度条件下的降酚动力学模式。结果表明,B3为蜡状芽孢杆菌(Bacilluscereus);苯酚浓度较低时,苯酚对菌株的生长基本不产生抑制作用,用Monod模型对B3降酚动力学过程进行拟合,其动力学参数Vmax=0.03h-1,Ks=25.53mg/L;苯酚浓度较高时,按照Andrews模型对B3降酚动力学过程进行非线性最小二乘曲线拟合,其动力学参数Vmax=0.08h-1,Ks=147.52mg/L,Ki=384.96mg/L。根据动力学方程,推论菌株B3降解对于浓度238.30mg/L的苯酚具有最佳降解效果。

References

[1]  王国华,任鹤云. 工业废水处理工程设计与实例. 北京:化学工业出版社,2005
[2]  张亚峰,叶姜瑜,秦艳. 高效降解焦化废水细菌的筛选. 水处理技术,2009,35(9):15-18 Zhang Y. F.,Ye J. Y.,Qin Y. Screening of efficient microorganism strain capable of degrading coking wastewater. Technology of Water Treatment,2009,35(9):15-18(in Chinese)
[3]  周江亚,李娟,于晓娟,等. 高浓度苯酚降解菌Candida tropicalis Z-04的鉴定及其对苯酚降解条件的优化. 环境污染与防治,2011,33(2):12-17 Zhou J.Y.,Li J.,Yu X.J.,et al. Identification of high-strength phenol degrading strain Candida tropicalis Z-04 and degradation condition. Environmental Pollution & Control,2011,33(2):12-17(in Chinese)
[4]  周倩倩,丁丛,王志平,等. 苯酚降解菌的筛选及其降解特性初探. 哈尔滨商业大学学报(自然科学版),2011,27(4):544-549 Zhou J.J.,Ding C.,Wang Z.P.,et al. Isolation of phenol-degrading strain and pretimonary studies on its degrading characteristics. Journal of Harbin University of Commerce(Natural Sciences Edition),2011,27(4):544-549(in Chinese)
[5]  Rothmel R. K.,Aldrich T. L.,Houghton J. E.,et al. Nucleotide sequencing and characterization of Pseudomonas putida catR:A positive regulator of the catBC operon is a member of the LysR family.Journal of Bacteriol.,1990,172(2):922-931
[6]  Muftah H. E.,Shaheen A. A.,Souzan M. Biodegradation of phenol by Pseudomonas putida immobilized in polyvinyl alcohol (PVA) gel. Journal of Hazardous Materials,2009,164(2-3):720-725
[7]  Handan U.,Ergun.,Alper N. Phenol biodegradation in a batch jet loop bioreactor (JLB):Kinetics study and pH variatio. Bioresource Technology,2010,101(9):2965-2971
[8]  国家环境保护总局.水和废水监测分析方法(第4版). 北京:中国环境科学出版社,2006
[9]  陈明,张维,徐玉泉,等. 醋酸钙不动杆菌PHEA-2对苯酚的降解特性研究. 中国环境科学,2011,21(3):226-229 Chen M.,Zhang W.,Xu Y. Q.,et al. The research on phenol-degrading character of acinetobacter calcoaceticus PHEA-2. China Environmental Science,2011,21(3):226-229(in Chinese)
[10]  Lee S. G.,Hung S. P. Removal and bioconversion of phenol in wasterwater by z-thermostable β-tryrosion. Enzyme and Microbial Technology,1996,19(3):374-377
[11]  Semple K. T.,Cain R. B. Biodegradation of phenol by the alga Ochrornonus danica. Appl. Environ. Microbiol.,1996,162(4):1256-1273
[12]  Xu Y. Q.,Zhang W. The study on the ultrastructure of some phenol degrading strains. Acta Agriculturae Nucleatae Sinica,2002,16(6):366-369
[13]  陈春,李文英,吴静文,等. 焦化废水中苯酚降解菌筛选及其降解性能. 环境科学,2012,33(5):1687-1692 Chen C.,Li W. Y.,Wu W. J.,et al. Screening and characterization of phenol-degrading bacteria from coking wasterwater. Environmental Science,2012,33(5):1687-1692(in Chinese)
[14]  沈萍,范秀荣,李广武. 微生物学实验(第3版). 北京:高等教育出版社,1999
[15]  Wang S. J., Loh K. C. Modeling the role of metabolic intermediates in kinetics of phenol biodegradation. Enzyme and Microbial Technology,1999,25(3-5):177-184
[16]  李淑彬,陈振军,丘李莉,等. 蜡状芽孢杆菌菌株Jp-A的分离鉴定及其降解苯酚特性. 应用生态学报,2006,17(5):920-924 Li S. B.,Chen Z.J.,Qiu L.L.,et al. Isolation and identification of Bacillus cereus strain Jp-A and its capability in phenol degradation.Chinese Journal of Applied Ecology,2006,17(5):920-924(in Chinese)
[17]  马溪平,艾娇,徐成斌,等. 耐低温苯酚降解菌的降解动力学研究. 环境保护科学,2009,35(5):18-21 Ma X.P.,Ai J.,Xu C.B.,et al. Study on kinetics of phenol biodegradation by low temperature-resistance strain. Environmental Protection Science,2009,35(5):18-21(in Chinese)
[18]  Arinjay K.,Shashi K.,Surendra K. Biodegradation kinetics of phenol and catechol using Pseudomonas putida MTCC-1194. Biochemical Engineering Journal,2005,22(2):151-159

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133