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PCR-DGGE研究亚硝化-电化学生物反硝化全自养脱氮工艺细菌多样性

Keywords: PCR-DGGE,脱氮,亚硝化-电化学生物反硝化,细菌多样性

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Abstract:

采用分子生物学手段PCR-DGGE技术对亚硝化-电化学生物反硝化全自养脱氮工艺细菌的多样性进行了研究。结果表明,亚硝化段内主要的细菌种群为相似于Nitrosomonassp.(AJ224410)和Nitrosomonassp.NM41(AF272421)的种群,相似性分别为97%和94%;电化学生物反硝化段细菌类群主要有β-proteobacteria类群、γ-proteobacteria类群和Chloroflexi类群。填料上生物膜细菌种群较底部泥水混合物丰富,两者细菌种群相似性为75%;底部泥水混合物样中存在与厌氧氨氧化菌Brocadiaanammoxidans(AF375994)相似性为93%的菌种,而填料上生物膜中存在与Thioalkalivibriosp.K90mix(EU709865)和Thiobacillusthioparus(AJ243144)相似性分别为94%、97%的菌种,其中Thiobacillusthioparus(AJ243144)是典型的硫自养反硝化菌,表明填料上生物膜中有大量的硫自养反硝化菌。

References

[1]  Chu H.,Fujii T.,Morimoto S.,et al.Community structure of ammonia-oxidizing bacteria under long-term application of mineral fertilizer and organic manure in a sandy loam soil.Applied and Environmental Microbiology,2007,73(2):485-491
[2]  陈谊,孙宝盛,黄兴,等.膜生物反应器中不同阶段微生物群落结构演变的研究.环境工程学报,2009,3(6):1023-1028 Chen Yi,Sun Baosheng,Huang Xing,et al. Study of microbial population succession in MBR at different stages. Chinese Journal of Environmental Engineering,2009,3(6):1023-1028(in Chinese)
[3]  Zhang B.,Sun B. S.,Ji M.,et al. Quantification and comparison of ammonia-oxidizing bacterial communities in MBRs treating various types of wastewater. BioresourceTechnology,2010,101(2):3054-3059
[4]  Riemann L.,Leitet C.,Pomier T.,et a1.The natire bacterioplankton comunity in the central baltic sea is influenced by freshwater bacterial species.Applied and Environmental Microbiology,2008,74(2):503-515
[5]  Liu Xinchun, Zhang Yu, Yang Min,et al.Analysis of bacterial community structures in two sewage treatment plants with different sludge properties and treatment performance by nested PCR-DGGE method. Journal of Environmental Sciences.2007,4(19):60-66
[6]  Fang H. H. P.,Zhang T.,Liu Y.Characterization of an acetate-degrading sludge without intracellular accumulation of polyphosphate and glycogen.Water Research,2002,36(3):3211-3218
[7]  董婧,宋玉栋,周岳溪,等. 处理含酚废水曝气生物流化床中丝状菌种群结构的解析.环境科学研究,2010,23(8):1068-1075 Dong Jing,Song Yudong,Zhou Yuexi,et al.Analysis of the Structure of filamentous microbial groups from aerated biological fluidized tank treating Phenolic wastewater. Research of Environmental Sciences,2010,23(8):1068-1075(in Chinese)
[8]  Murray A. E., Hollibaugh J. T., Orrego C. Phylogenetic composition of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments. Applied and Environmental Microbiology,1996,62(7):2676-2680
[9]  李军,杨秀山,彭永臻.微生物与水处理工程.北京:化学工业出版社,2002.404-405
[10]  Hellinga C.The SHARON process:An innovative method for nitrogen removal from ammonium-rich wastewater.Water Science and Technology,1998,37(9):135-142
[11]  袁林江,彭党聪,王志盈,等.短程硝化反硝化生物脱氮.中国给水排水, 2000,16(2):29-31 Yuan Linjiang,Peng Dangcong,Wang Zhiying,et al. Biological nitrogen removal by shortcut nitrification-denitrification.Chinese Water and Wastewater,2000,16(2):29-31(in Chinese)
[12]  王海燕,周岳溪,刘海涛,等.亚硝化电化学反硝化全自养工艺微生学及细菌多样性研究.环境科学学报,2010,30(2):327-339 Wang Haiyan,Zhou Yuexi,Liu Haitao,et al.Morphology and diversity of the bacteria in the complete autotrophic nitrite-nitrification and electrochemical bio-denitrification combined process. Acta Scientiae Circumstantiae,2010,30(2):327-339(in Chinese)
[13]  张伟华,王海燕,曾清如,等.CANNED工艺细菌种群结构变化的FISH研究.环境科学研究,2010,23(9):1185-1191 Zhang Weihua,Wang Haiyang,Zeng Qingru,et al.Study of bacterial community changes in CANNED process by FISH method. Research of Environmental Sciences, 2010,23(9):1185-1191(in Chinese)
[14]  Kuenen J. G., Jetten M. S. M. Extraordinary anaerobic ammonium-oxidizing bacteria. ASM News, 2001,67(9):456-463
[15]  Koeing A.,Liu L. H.Autotrophic denitrification of landfill leaehate using elemental sulfur. Water Science and Technology,1996,34(5-6):469-476
[16]  王海燕,葛建团,刘海涛,等.亚硝化/电化学生物反硝化全自养脱氮工艺研究.环境科学学报, 2007,27(3): 375-385 Wang Haiyan,Ge Jiantuan,Liu Haitao,et al. Complete autotrophic nitrite-nitrification and electrochemical bio-denitrification combined process. Acta Scientiae Circumstantiae,2007,27(3):375-385(in Chinese)
[17]  Zhou J. Z., Bruns M. A., Tiedje J. M. DNA Recovery from Soils of Diverse Composition. Appl Environ Microbiol,1996,62(2):316-322
[18]  肖勇, 杨朝晖, 曾光明,等. PCR-DGGE研究处理垃圾渗滤液序批式生物膜反应器(SBBR)中的细菌多样性.环境科学, 2007, 28(5): 1095-1101 Xiao Yong,Yang Zhaohui,Zeng Guangming,et al.Bacterial diversity in sequencing batch biofilm reactor (SBBR) for landfill leachate treatment using PCR-DGGE.Environmental Scinece,2007,28(5): 1095-1101(in Chinese)

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