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水解溶菌酶污泥减量过程中的微生物群落变化

Keywords: 污泥减量,水解溶菌酶,SBR,PCR-DGGE

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Abstract:

利用水解溶菌酶对SBR系统中的剩余污泥进行减量。通过与未加水解溶菌酶的相同系统对比,研究了水解溶菌酶作用下的SBR系统中剩余污泥的减量效果与微生物群落结构的变化。结果表明,在50d的运行期内,水解溶菌酶作用下的SBR系统中剩余污泥减量总计达到76.3%,同时该系统对COD与TN的平均去除率分别为88.2%与53.8%。通过PCR-DGGE分析可知,随着运行时间的增加两系统微生物群落结构的差异逐步明显,SBR系统中原有的部分优势微生物在水解溶菌酶的作用下逐渐减弱。另外,对微生物群落的部分优势细菌进行克隆测序和系统发育树分析,通过鉴定获得的7条细菌的16SrDNA序列,它们分别与放线菌和杆菌同源性在97%以上。

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