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降解聚丁二酸丁二醇酯菌株的诱变选育及降解性能

Keywords: 聚丁二酸丁二醇酯(PBS),尖孢镰刀菌,降解性能,诱变,酶活性

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Abstract:

诱变选育是基因资源开发的重要手段之一,为了对降解聚丁二酸丁二醇酯(PBS)菌株的降解性能进行改良,选择具有一定降解PBS能力的尖孢镰刀菌(Fusariumoxysporum)DSCF-8为出发菌株,分别研究了其在5种诱变途径下的突变效应及最佳诱变条件,测定了突变菌株D8B14在不同条件下的降解性能。结果表明,复合诱变途径的高效降解菌株获得率大于单因子诱变途径,分别达到了4.55%和1.30%,但突变株的降解特性遗传不稳定;硫酸二乙酯单因子诱变效应大于紫外线诱变和微波诱变,且得到的突变株较复合诱变得到的菌株具有更强的遗传稳定性,最佳诱变条件为5%的浓度处理40min。硫酸二乙酯诱变得到的突变株D8B14传代稳定且降解性能优异,其对PBS薄膜的降解率较出发菌株提高了34.49%,粗酶液酶活性提高率为23.86%。该突变株在35℃条件下仍表现出较高的降解能力,对不同的初始pH条件亦具有更强的耐受能力,尤其在pH4.0或pH9.0的酸性和碱性条件下,降解率分别提高了13.26%和27.04%。

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