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膨化预处理玉米秸秆的还原糖酶解工艺

Keywords: 玉米秸秆,膨化预处理,酶解,还原糖,正交实验

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Abstract:

应用膨化技术对玉米秸秆进行预处理。在单因素实验基础上,以还原糖转化率为影响值设计正交实验,研究温度、pH、液固比、酶浓度及酶解时间五因素对纤维素酶解过程的影响。得出玉米秸秆糖化酶解最佳工艺条件为:温度48℃,pH4.5,液固比8∶1,酶浓度36.0U/g,酶解时间25h;在此工艺条件下还原糖转化率达到28.98%。扫描电镜表征对比观察可看出,膨化后玉米秸秆纤维素酶解充分;结合红外光谱对各组分特征基团分析表明,膨化后的玉米秸秆酶解的纤维素基团特征峰变化更为明显。

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