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毒性微生物传感器的研制及应用

Keywords: 微生物传感器,毒性测试,印染废水

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Abstract:

以从土壤中分离、筛选出的X4菌株作为分子识别元件,采用夹层法制备微生物膜并与极谱型氧电极组成了呼吸抑制型毒性微生物传感器。实验确定了传感器的最佳工作条件:温度为30℃,pH范围为7.0~7.5;以HgCl2作为参比毒物时,微生物传感器标准曲线为y=1.05x+0.21,线性相关系数为0.9933,线性范围为0.1~1.97mg/L,相对标准偏差为1.8%。用该微生物传感器对某印染废水的毒性进行测试,该废水的毒性相对于HgCl2的浓度为1.69mg/L,属于剧毒,与发光细菌法进行比对,测定结果具有一致性。

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