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不适宜生长条件对混菌降解苯胺的影响

Keywords: 苯胺,Comamonas,sp.TZ1,Pseudomonas,sp.JH1混合菌,耐受能力,生物降解

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Abstract:

分别从台州和衢州某化工厂的好氧池中分离筛选得到2株苯胺降解菌TZ1和JH1,经16SrDNA测序鉴定为Comamonassp.TZ1和Pseudomonassp.JH1,均具有较强的苯胺降解能力,培养24h后,可使初始浓度为800mg/L的苯胺去除率达到96.4%~98.4%。在此基础上,按体积比1:1将2株菌液进行混合构建了混合菌体系,进而对比考察了苯胺初始浓度、pH、盐度和重金属等环境因子对单一菌和混合菌生长量及降解苯胺效果的影响,重点探讨混合菌对不适宜生长环境的适应性及其对苯胺的降解特性。通过单一菌和混合菌对比实验发现,在适宜苯胺初始浓度、pH和盐度条件下,混合菌的生长量略高于单一菌;在不适宜生长的高浓度苯胺、pH和盐度条件下,混合菌也表现出了更强的适应性和苯胺矿化能力。Zn2+和Cr6+耐受实验则表明,对于Cr6+,混合菌表现出了更强的耐受能力,而对于Zn2+并没有表现出更强的耐受能力。

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