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科技导报  2015 

利用SSR标记正确判断小麦杂交种中的杂株

DOI: 10.3981/j.issn.1000-7857.2015.16.005, PP. 39-45

Keywords: 杂交小麦,SSR,标记,杂株

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Abstract:

正确判断杂交种中混有的杂株,是准确鉴定小麦杂交种种子纯度的前提。在分析180份杂交组合的基础上,以“京麦1100”为例,提出了利用SSR标记正确判断小麦杂交种中杂株的方法1)筛选双亲间有多态性的引物不少于5对;2)利用该引物检测亲本及杂交种的基因型,并按照杂交种基因型记录原则记录每个个体的基因型;3)正确判断真实杂交种基因型不论亲本是否存在非纯合位点现象,只要某个体同时具备父母本的基因型,均视为真实杂交种的基因型;4)判断杂株当某个体在多个SSR位点上与真实杂交种基因型不同时,判断为杂株。

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