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科技导报  2015 

高尿酸血症大鼠模型血清代谢组学研究

DOI: 10.3981/j.issn.1000-7857.2015.16.012, PP. 77-80

Keywords: 高尿酸血症,代谢组学,大鼠模型,1H-NMR,

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Abstract:

通过检测高尿酸血症大鼠血清代谢谱的变化,探讨代谢组学在高尿酸血症研究中的应用。将20只清洁级健康SD(Sprague-Dawley)大鼠随机分为高尿酸血症模型组(10只)和空白对照组(10只),每日采用酵母膏(YEP)21g·kg-1·d-1给予喂养,氧氢酸钾(OA)以200mg-1·kg-1·d-1腹腔注射1次,建立高尿酸血症大鼠模型。第28日收集空白对照组和模型组大鼠血清,进行1H-NMR谱测定和主成分分析。与空白对照组大鼠相比,模型组大鼠血清中糖蛋白、丙酮酸、丙氨酸、异亮氨酸、亮氨酸显著增加,而甲酸浓度及极低密度脂蛋白、不饱和脂肪酸、丙酮显著降低。由此得出,高尿酸血症大鼠血清代谢组学发生明显变化,血浆代谢组学在一定程度上反映高尿酸血症大鼠的病理生理变化。

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