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科技导报  2015 

蜂胶黄酮Pinobanksin-3-acetate对结肠癌HCT-116细胞增殖及凋亡的影响

DOI: 10.3981/j.issn.1000-7857.2015.09.012, PP. 69-73

Keywords: Pinobanksin-3-acetate,HCT-116,细胞株,MTT,比色法,细胞凋亡

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Abstract:

为探讨蜂胶黄酮(Pinobanksin-3-acetate,PB3A)对结肠癌HCT-116细胞增殖及细胞凋亡的影响,采用四甲基偶氮唑盐(MTT)比色法,检测不同浓度、不同时间PB3A、5-氟尿嘧啶(5-FU)及两种药物联合作用对HCT-116细胞生长所产生的影响,倒置显微镜观察细胞的形态学变化特点,AnnexinV-FITC/PI双染色、流式细胞仪检测药物作用24h后的细胞凋亡率。结果显示,PB3A对HCT-116细胞增殖有明显的抑制作用,并呈浓度和时间依赖性。其抑制活性与5-FU几乎没有显著性差异,联合用药具有协同效应;在一定剂量范围内,可见凋亡细胞明显增多。流式细胞仪分析结果表明,细胞凋亡率明显上升,呈剂量依赖性。PB3A对HCT-116细胞具有明显的增殖抑制和诱导细胞凋亡作用。

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