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科技导报  2015 

耐低温降解生物质废弃物复合菌剂的混料设计优化

DOI: 10.3981/j.issn.1000-7857.2015.08.003, PP. 24-29

Keywords: 混料设计,复合菌剂,纤维素酶,耐低温,生物质废弃物

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Abstract:

为了筛选制备耐低温高效降解农业生物质废弃物的复合菌剂,在已获得的耐低温木质纤维素降解菌群A25-3的基础上,采用混料设计(mixturedesign)对复合菌比例进行优化,通过测定菌群A25-3、酿酒酵母(Saccharomycescerevisiae)和绿色木霉(Trichodermaviride)不同比例搭配对纤维素酶(CMCase、滤纸酶和外切葡聚糖酶)、蛋白酶和α-淀粉酶活性的影响,建立了各菌株(群)搭配比例与试验指标之间的回归方程。使用DesignExpert8.0软件的优化功能对满足所有期望的响应值进行优化,得到复合菌的最优配比(质量分数)为绿色木霉37.24%,菌群A25-367.76%。对该最优比例进行验证试验,得到的结果与预测值基本一致。秸秆降解试验表明,固体复合菌剂可高效降解秸秆,有效提高堆肥体系的温度,在低温环境下的堆肥和秸秆腐熟具有很好的应用前景。

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