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科技导报  2015 

维吾尔族内皮型一氧化氮合酶基因4a/bVNTR多态性分布特征

DOI: 10.3981/j.issn.1000-7857.2015.06.017, PP. 101-106

Keywords: 维吾尔族,内皮型一氧化合酶基因,基因多态性,数目可变性串联重复序列

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Abstract:

应用聚合酶链式反应(PCR)-琼脂糖凝胶电泳技术,检测99例(男性38例,女性61例)无血缘关系的维吾尔族健康人群的内皮型—氧化氮合酶(endothelialnitricoxidesynthase,eNOS)基因第4内含子数目可变性串联重复序列(4a/bVNTR)多态性,探讨eNOS基因4a/bVNTR多态性在中国维吾尔族健康人群中的分布特征。结果发现,99例健康维吾尔族人群中,aa基因型频率为2.02%(4例),ab基因型频率为22.22%(22例),bb基因型频率为75.76%(75例)。男女两组基因型和等位基因的分布差异不显著(P=0.194,P=0.382,P>0.05)。aa、ab、bb、ab+bb基因型的各种生理生化指标用单因素方差分析进行比较,不同基因型的生理生化指标之间的差异无统计学意义(P>0.05)。维吾尔族eNOS基因4a/bVNTR多态性与国内外各民族群体进行比较,维吾尔族人群eNOS基因aa+ab基因型频率显著低于河南汉族、中国台湾汉族、北方汉族和巴西黑人(P0.05),群体具有代表性。由此得出,维吾尔族eNOS基因4a/bVNTR多态性与国内外的一些民族间存在显著性差异。

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