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科技导报  2014 

曲霉属产毒真菌DNA的制备及其毒素相关基因aflR的PCR检测

DOI: 10.3981/j.issn.1000-7857.2014.14.004, PP. 31-34

Keywords: 产毒真菌,DNA,提取,黄曲霉毒素,RCR,检测

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Abstract:

研究了用改良的CTAB法提取曲霉属产毒真菌DNA的方法,并与试剂盒提取法进行比较。经过改良的CTAB法能够成功提取出曲霉属产毒真菌的DNA,且浓度和纯度均可达到PCR扩增的标准。根据合成黄曲霉毒素的aflR基因设计两对引物,运用PCR方法进行扩增鉴定,结果表明黄曲霉、寄生曲霉和溜曲霉基因中可检出aflR基因,烟曲霉和杂色曲霉中并未检出,达到对黄曲霉毒素产生菌进行鉴定的目的。这种方法可为产毒真菌的快速检测提供参考。

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