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科技导报  2014 

乳腺癌患者血浆和尿液的1H-NMR代谢组学

DOI: 10.3981/j.issn.1000-7857.2014.13.010, PP. 58-63

Keywords: 乳腺癌,代谢组学,代谢标志物体系

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Abstract:

为探讨乳腺癌患者血浆、尿液代谢组学变化,建立早期诊断的代谢标志物体系。选取乳腺癌患者45例,健康对照30例,获得血浆1H-NMR谱图,对所得自由衰减信号进行分段积分、数据归一化后用正交偏最小二乘法判别分析(OPLS-DA)进行模式识别。与健康人群相比,乳腺癌患者血浆中多种氨基酸含量下降,包括亮氨酸、缬氨酸、苯丙氨酸、酪氨酸、丙氨酸、组氨酸、甘氨酸、脂类(LDL)、β-葡萄糖、乳酸、胆碱、肉碱、肌醇显著减少,差异具有统计学意义(P<0.05);血浆糖蛋白、丙二酸、乙酰乙酸、β-羟丁酸、丙酮显著增多,差异具有统计学意义(P<0.05)。与健康人相比,乳腺癌患者尿液中异亮氨酸、缬氨酸、谷氨酸、组氨酸、二甲胺、马尿酸、甜菜碱、牛磺酸、琥珀酸、柠檬酸、肌酸、肌酸酐减少,差异具有统计学意义(P<0.05);乳酸增多,差异具有统计学意义(P<0.05)。通过乳腺癌患者的代谢组学的变化,建立可以用于乳腺癌早期诊断的血浆、尿液代谢标志物体系。

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