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科技导报  2014 

α-Synuclein蛋白过表达对小鼠纹状体的影响

DOI: 10.3981/j.issn.1000-7857.2014.07.002, PP. 22-26

Keywords: 帕金森病,α-synuclein,蛋白,纹状体,氧化应激,儿茶酚异喹啉类物质

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Abstract:

帕金森病是世界第二大老年神经退行性疾病,致病机理极为复杂。α-synuclein(α-syn)是帕金森病主要病理特征的路易小体的主要组成成分,其突变基因α-synA30P也与部分家族性帕金森相关。通过对过表达野生型人源α-synWT及其突变体α-synA30P蛋白的转基因小鼠的行为学检验、脑部纹状体中氧化应激水平以及儿茶酚胺异喹啉物质水平的检测,研究过表达α-syn蛋白对小鼠纹状体产生的影响。结果显示转基因小鼠模型与正常鼠相比,其协调能力明显下降,纹状体与全脑的比例显著降低。同时,模型鼠脑中的氧化应激水平与儿茶酚胺异喹啉物质的表达水平均显著升高。研究结果说明,α-syn蛋白及其突变体的过表达会引起小鼠脑部纹状体中氧化应激水平与儿茶酚异喹啉物质表达水平的升高,从而导致纹状体组织严重损伤。

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