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科技导报  2015 

桑叶金花茶中酚类物质动态变化及其抗氧化活性

DOI: 10.3981/j.issn.1000-7857.2015.07.016, PP. 95-99

Keywords: 固体双向发酵,桑叶金花茶,总黄酮,总酚酸,体外抗氧化

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Abstract:

为探讨桑叶固体双向发酵过程中水溶性酚类物质含量的动态变化及其抗氧化活性,应用三氯化铝比色法、Folin-酚法测定桑叶固体双向发酵物中水溶性总黄酮及总酚酸含量,并以清除1-1-二苯基-2-三硝基苯肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+)、铁还原能力3个指标,评价桑叶固体双向发酵过程中体外抗氧化活性的变化情况。结果发现,随着发酵时间的延长,桑叶固体双向发酵物中水溶性总黄酮、总酚酸均呈先增加后减少的趋势。其中发酵14d水溶性黄酮质量分数最多为2.16mg/g,20d水溶性总酚酸质量分数最多为1.48mg/g。对于体外3个抗氧化指标而言,整体表现为14d>7d>11d>20d>40d。

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