全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
科技导报  2015 

维药异常黑胆质成熟剂对人肝癌HepG2细胞生物行为和Rho/ROCK信号通路的影响

DOI: 10.3981/j.issn.1000-7857.2015.04.015, PP. 85-90

Keywords: 异常黑胆质成熟剂,Rho/ROCK,肝癌细胞(HepG2)

Full-Text   Cite this paper   Add to My Lib

Abstract:

为探讨维药异常黑胆质成熟剂(ASM)对人肝癌细胞(HepG2)增殖、侵袭转移的影响及Rho/ROCK信号传导通路相关蛋白表达影响,采用四甲基偶氮唑蓝(MMT)法检测不同浓度ASM(10、20、25、50mg/mL)和10μmol/LY-27632作用24、48、72h后,对HepG2细胞增殖的影响;采用扫描电镜技术和细胞侵袭实验测定ASM不同剂量组和10μmol/LY-27632作用24h癌细胞侵袭运动能力,WesternBlot检测ASM不同剂量组和10μmol/LY-27632作用24h癌细胞RhoA、ROCK1、ROCK2的表达。结果显示,ASM对肝癌细胞增殖有明显抑制作用,且表现为有明显的剂量效应关系在10、20mg/mLASM剂量组,ASM药物作用24、48、72h后,HepG2细胞增殖抑制作用随时间的延长而抑制增加,而25、50mg/mL剂量组,ASM抑制细胞增殖作用不明显;ASM抑制瘤细胞侵袭运动能力,扫描电镜结果显示ASM抑制肿瘤细胞伪足的生长,ASM中高剂量组ROCK1、ROCK2的表达明显降低,RhoA表达无明显变化。由此推论,ASM对人肝癌细胞生长增殖和侵袭运动能力有抑制作用,其机制可能与ROCK酶表达降低有关。

References

[1]  哈木拉提·吾甫尔. 维吾尔医异常黑胆质新论[M]. 乌鲁木齐: 新疆人民出版社, 2009: 1-9. Halmurat Upur. New conception on the theory of abnormal Savda in traditional Uyghur medicine[M]. Xinjiang: Xinjiang Pepole's Press, 2009: 1-9.
[2]  玛依努尔·艾力, 哈木拉提·吾甫尔. 维吾尔药异常黑胆质成熟剂总黄酮逆转肝癌细胞耐药性的作用[J]. 中国中医药信息杂志, 2007, 14 (9): 31-33. Mayinur Ali, Halmurat Upur. The reverse effect of uygur medicine ASM on the drug resistance cells of liver cancer[J]. Chinese Journal of Information on TCM, 2007, 14(9): 31-33.
[3]  胡汉华, 盛磊, 哈木拉提·吾甫尔, 等. 维药异常黑胆质成熟剂的抗肿瘤作用及其对细胞迁移的影响[J]. 科技导报,2011, 29(3): 62-65. Hu Hanhua, Sheng Lei, Halmurat Upur, et al. Anti-cancer effects of uighur medicine abnormal savda munzip and its influence on cell migration[J]. Science & Technology Review, 2011, 29(3): 62-65.
[4]  阿不都热依木·玉苏甫, 哈木拉提·吾甫尔, 吐尔洪·卡迪尔, 等. 异常黑胆质成熟剂乙酸乙酯萃取物对HepG2细胞生长和凋亡及相关基因调控的研究[J]. 中国药科大学学报, 2006,37(2): 161-164. Abdirymu Yusup, Halmurat Upur, Turghun Kadir, et al. Effect of abnormal savda Munziq ethyl acetate extract on the proliferation, apoptosis and correlative gene expression in human hepatoma (HepG2) cells[J]. Journal of China Pharmaceutical University, 2006, 37(2): 161-164.
[5]  王国宁, 李华, 陈珂瑶, 等. 异常黑胆质成熟剂总酚与顺铂、多西他赛联用对宫颈癌HeLa细胞增殖和凋亡的影响[J]. 山东医药, 2011, 51 (22): 22-24. Wang Guoning, Li Hua, Chen Keyao, et al. Effects of total phenolics from abnormal savda munziq combined with cisplation, ocetaxdon HeLa cells proliferation and apoptosis[J]. Shandong Medical Journal, 2011, 51 (22): 22-24.
[6]  张阿丽, 马丁. 细胞迁移调控因子Rho与肿瘤转移[J]. 国外医学生理病理科学与临床分册, 2003, 23(4): 365-367. Zhang Ali, Ma Ding. Cell migration control factor Rho and tumor metastasis[J]. Foreign Medical Sciences·Section of Pathophysiology and Clinical Medicine, 2003, 23(4): 365-367.
[7]  邵静, 王红兵, 杨力, 等. Rho蛋白对肿瘤细胞骨架活动及生长调节的影响[J]. 生物医学工程学杂志, 2008, 25(6): 1462-1465. Shao Jing, Wang Hongbing, Yang Li, et al. Effects of Rho proteins on the cytoskeleton activity and the growth regulation in tumor Cells[J]. Journal of Biomedical Engineering, 2008, 25(6): 1462-1465.
[8]  王德盛, 窦科峰, 李开宗, 等. Rho/Rho激酶对肝癌细胞体外侵袭转移作用的研究[J]. 中华普通外科杂志, 2005, 20(4): 245-247. Wang Desheng, Dou Kefeng, Li Kaizong, et al. Studies on hepatic carcinoma cell invasion in vitro through Rho/Rho kinase pathway[J]. Chinese Journal of General Surgery, 2005, 20(4): 245-247.
[9]  Kamai T, Tsujii T, Arai K, et al. Significant association of Rho/ROCK pathway with invasion and metastasis of bladder cancer[J]. Clinical Cancer Research, 2003, 9(7): 2632-2641.
[10]  Aikemu A, Yusup A, Umar A, et al. The impact of the Uighur medicine abnormal savda munziq on antitumor and antioxidant activity in a S180 and Ehrlich ascites carcinoma mouse tumor model[J]. Pharmacogn Magazine, 2012, 8(30): 141-148.
[11]  杨靖亚,王锡昌,刘梅芳,等. 表没食子儿茶素没食子酸酯通过下调 GTP结合蛋白RhoA的表达抑制癌细胞的浸润和转移[J]. 中国临床药理学与治疗学, 2006; 11(7): 729-734. Yang Jingya, Wang Xichang, Liu Meifang, et al. (-)-Epigallocatechin-3-gallate suppresses migration and invasion of cancer cell by decreasing expression of small GTP-binding protein RhoA[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(7): 729-734.
[12]  Liu N,Bi F,Pan Y, et al. Reversal of the malignant phenotype of gastric cancer cells by inhibition of RhoA expression and activity[J]. Clinical Cancer Research, 2004, 10(18 Pt 1): 6239-6247.
[13]  罗元. 细胞迁移运动相关因子与肿瘤浸润转移的关系[J]. 中国医学文摘(肿瘤学), 2004, 18(4): 58-59. Luo Yuan. The relation of the factors related cellular migration and tumor invasion[J]. Journal of Chinese Medical Abstracts (Oncology), 2004, 18(4): 58-59.
[14]  王雪, 王维莉, 任雨, 等. RhoA介导的细胞骨架在肿瘤发生发展中的作用[J]. 中国细胞生物学学报, 2014, 36(2): 267-273. Wang Xue, Wang Weili, Ren Yu, et al. The function of cytoskeleton mediated by RhoA during tumorigenesis and development[J]. Chinese Journal of Cell Biology, 2014, 36(2): 267-273.
[15]  方雷, 吴海滨, 陈晓岗. RhoA基因沉默对胃癌MGC-803细胞增殖和迁移能力的影响[J]. 肿瘤学杂志, 2012, 18(2): 28-32. Fang Lei, Wu Haibing. Effect of RhoA gene silencing on proliferation and migration of gastric cancer cell line MGC-803[J]. Journal of Oncology, 2012, 18(2): 28-32.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133