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南京小麦梭条花叶病毒RT-PCR的分子鉴定

DOI: 10.7685/j.issn.1000-2030.2001.02.009, PP. 37-40

Keywords: 小麦梭条花叶病毒,RT-PCR,分子鉴定

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Abstract:

采自江苏省南京市郊区的小麦病毒病样品提纯后,经SDS聚丙烯酰胺凝胶电泳,结果表明,病毒外壳蛋白(CP)为单一组分,相对分子质量为365×103。经12g?dL-1琼脂糖凝胶电泳检测,病毒核酸有两个组分,即RNA1和RNA2。根据AnbeSohn发表的小麦梭条花叶病毒RNA13′端部分核苷梭序列,设计一对引物,以提纯样品的RNA1为模板,经RTPCR扩增出包含CP基因在内的17kb的特异性目的片段,据此鉴定所采集的毒原为小麦梭条花叶病毒(WSSMV)。

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