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苏太猪肌内脂肪沉积相关基因SRC-1的差异显示反转录PCR鉴定

DOI: 10.7685/j.issn.1000-2030.2009.03.023, PP. 126-129

Keywords: 差异显示反转录PCR,肌内脂肪,实时荧光定量PCR,细胞核受体共激活因子1基因(SRC-1),苏太猪

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Abstract:

采取90头180日龄去势苏太猪的背最长肌组织测定肌内脂肪含量,从中选取肌内脂肪含量差异极显著(P<0.01)的最高和最低各6头组成RNA池,采用差异显示反转录PCR(DDRT-PCR)鉴定两组间差异表达的基因,结果共获得14条表达序列标签(expressedsequencetags),即EST序列,通过GenBank比对发现其中3条为已知序列。为进一步验证这3个已知基因与肌内脂肪之间的关系,从90个个体中选取肌内脂肪最高和最低各15个个体进行实时定量PCR检测。结果表明,细胞核受体共激活因子1基因(SRC-1)mRNA表达水平与肌内脂肪含量存在负相关关系(r=-0.38,P<0.05)。提示SRC-1蛋白有可能参与了肌内脂肪沉积的调节。

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