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6-苄基腺嘌呤对水稻颖花分化影响机制的研究

DOI: 10.7685/j.issn.1000-2030.2009.03.002, PP. 8-13

Keywords: 水稻,颖花分化,6-苄基腺嘌呤(6-BA),水稻细胞分裂素氧化酶基因(OsCKX2)

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Abstract:

以常规籼稻品种扬稻6号为材料,在盆栽条件下,于生长锥伸长期外喷6-苄基腺嘌呤(6-BA)后,定期取样,测量穗部细胞分裂素(cytokinins,CKs)含量,同时采用RT-PCR检测水稻穗分化过程中细胞分裂素氧化酶基因(OsCKX2)的表达模式,考察水稻穗部性状。结果发现6-BA处理促进水稻穗长发育并显著提高每穗枝梗和颖花数,分化枝梗数和总颖花数分别为60和255.4,比对照分别提高了10%和30.5%。穗部细胞分裂素含量的增加,刺激了更多颖花分化。6-BA处理的水稻穗部OsCKX2表达时间提前,但表达量下降。认为水稻穗分化始期喷施6-BA可降低穗部OsCKX2的表达强度,提高穗部细胞分裂素含量,对枝梗和颖花分化产生促进作用,为形成大穗提供了先决条件。

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