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柔嫩艾美耳球虫DNA疫苗的构建及其在鸡肌肉组织中的表达

DOI: 10.7685/j.issn.1000-2030.2008.02.021, PP. 101-104

Keywords: 柔嫩艾美耳球虫,DNA疫苗,pEtK2基因,IL-2基因

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Abstract:

利用DNA重组技术,分别将柔嫩艾美耳球虫(Eimeriatenella)pEtK2基因单独或与鸡白细胞介素2基因(IL-2)串联克隆到DNA疫苗载体pVAX1或pcDNA4.0(c)上,构建了pVAX1-pEtK2、pcDNA4.0(c)-pEtK2、pVAX1-pEtK2-IL-2、pcDNA4.0(c)-pEtK2-IL-2DNA疫苗。酶切鉴定正确后分别将重组质粒胸肌注射14日龄雏鸡,1周后取注射部位肌肉组织,用RT-PCR和Western-blotting法检测保护性抗原的表达情况。结果表明,抗原基因pEtK2和pEtK2-IL-2串联基因均能在注射部位肌肉组织中成功表达。

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