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水牛慢性实验感染肝片吸虫对安替比林代谢动力学的影响

DOI: 10.7685/j.issn.1000-2030.2000.04.020, PP. 81-84

Keywords: 水牛,肝片吸虫,安替比林,清除率,代谢,药物动力学

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Abstract:

5头健康雄性去势水牛(2~3岁、体重300~500kg),经粪便检查和DotELISA检测确认无肝片吸虫感染。每头每天口服60个肝片吸虫囊蚴,连续20d,共感染1200个囊蚴,用以研究水牛慢性感染(少量多次感染)肝片吸虫对安替比林代谢动力学的影响。每周定时由颈静脉采血,测定血清酶水平变化。于感染前、感染后急性期及慢性期进行安替比林动力学试验。由颈静脉瘘管收集血样,用尿液收集器收集尿样,用HPLC法分析血浆安替比林动力学参数及尿安替比林清除率。结果表明水牛慢性实验性感染肝片吸虫呈亚临床状态,AP的血浆及尿代谢物清除率在急性期分别下降了48%和6191%,慢性期逐步恢复。

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