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传染性支气管炎病毒地方分离毒株S1基因的RT-PCR方法研究

DOI: 10.7685/j.issn.1000-2030.2000.03.022, PP. 85-88

Keywords: 传染性支气管炎病毒,反转录,地方分离株,S1基因

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Abstract:

以传染性支气管炎病毒(IBV)标准毒株M41为材料,根据Genbank公开序列自行合成一对特异引物,用于扩增IBV的完整S1基因。建立了针对IBV基因组RNA特点的RTPCR方法。对反应体系中的重要反应物Mg2+、dNTP和引物浓度进行优化,确定其浓度值分别为15mmol/L,200μmol/L和40μmol/L。使用此反应体系对国内IBV地方分离毒株JS/95/03,SD/97/01等10多个毒株的S1基因进行扩增,均扩增出预期17kb的完整S1基因,说明此方法对于IBV的S1基因扩增具有一定的通用性

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