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小麦叶片中H2O2的3种测定方法比较

DOI: 10.7685/j.issn.1000-2030.2000.02.025, PP. 101-104

Keywords: 小麦叶片,过氧化氢,测定方法

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Abstract:

比较研究了目前正在应用的植物叶片中H2O2含量定量测定的3种方法,结果表明,Ti(IV)H2O2比色法有严重背景干扰,测定结果偏高;而5%TCA(三氯乙酸)提取,活性炭脱色,Ti(IV)PARH2O2比色法中,活性炭吸附了部分H2O2,测定结果偏低;改用萃取脱色时,有高回收率(95%以上),干扰小的特点,适宜植物叶片中微量H2O2的定量测定

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