全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

鸡源大肠杆菌1型菌毛结构基因(pilA)克隆

DOI: 10.7685/j.issn.1000-2030.2000.02.021, PP. 85-88

Keywords: 鸡大肠杆菌,1型菌毛,结构基因,克隆

Full-Text   Cite this paper   Add to My Lib

Abstract:

根据人源大肠杆菌1型菌毛结构基因(pilA)DNA序列,在其保守区设计了1对带有BamHI/HindⅢ酶切位点的引物,经PCR扩增,从24株鸡源大肠杆菌致病株染色体中得到21个大小约657bp的阳性产物,经酶切、连接、转化及筛选,得到3种来自不同血清型鸡源大肠杆菌(O1、O78及O88)PCR产物的阳性克隆。经核酸序列测定,确认所克隆的外源基因为1型菌毛pilA基因

References

[1]  Orndorff P E,Falkow S.Nucleotride sequence of pilA,the gene encoding the strutural component of type 1 pili in Escherichia coli[J].Journal of Bacteriology.1985,162:454-457
[2]  Hull R A,Gill R E,Hsu P.Construction and expression of recombinat plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate[J].Infection and Immunity.1981,33:933-938
[3]  Naveh M W,Zusman T,Skutelsky E.Adherence pili in avian strains of Escherichia coli:effect on pathogenicity[J].Avian Diseases.1984,28:651-661
[4]  Dozois C M,Fairbrother J M,Harel J.pap-and pil-related DNA sequences and other virulence determinats associated with Escherichia coli isolated from septicemic chickens and turkeys[J].Infection and Immunity.1992,60:2648-2565
[5]  Dozois C M,Fairbrother J M,Harel J.Expression of P and type 1(F1)fimbriae in pathogenic Escherichia coli from poultry[J].Veterinary Microbiology.1995,45:297-309
[6]  Suwanichkul A,Panigrahy B.Biological and immunological characterization of pili of Escherichia coli serotype O1,O2and O88 pathogenic to poultry[J].Avian Diseases.1986,30:781-786
[7]  丁?萨布鲁克,EF 弗里奇,J 曼尼阿蒂斯.分子克隆实验指南[M].北京:北京科学技术出版社.1992:55-56
[8]  van Die I,van Hoekstra G W,et al.Type 1C fimbriae of a uropathogenic Escherichia coli strain:cloning and characterization of the genes involved in the expression of the 1C antigen and nucleotide sequence of the subunit gene[J].Gene.1985,34:187-196

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133